Help file for results table
No. of ATG from 5' end
A sequence may have many ATG triplets. This information tells you the
number of an ATG's occurence relative to the 5' end
Reliability
Although the program works using the score from a linear discriminant function
this is difficult for users to interpret. So, instead of showing the user the
ldf score, we calculate a reliability score that
states the number of times predictions are normally correct when a particular
ldf score has been achieved. Specificallyi, the estimate of reliability is derived from
our tests using a non-redundant set of 660 sequences.
For example, a reliability
of 0.12 means that in our tests the reliability of the ldf score
was only 12%
and should therefore be treated with the upmost caution.
Frame
Tells the user in which of the three possible frames each ATG was located
Identity to Kozak rule
Kozak was the first to note that real initiation codons have a
strong preference for the pattern A/GXXATGG. This section of the
table shows the user how close a predicted ATG is to this pattern
Start (bp)
The number of the nucleotide at which the translation starts.
ie the A of the initiating ATG.
Finish (bp)
The number of the nucleotide at which the translation finishes.
This could be the end of the input sequence. See Stop codon found?
ORG length (aa)
The length of the predicted ORF in amino acids. This is (Finish-Start)/3.
Stop codon found?
Tells the user whether a termination codon was found or whether the
program read through to the end of the input sequence